Therefore, we used animal model to evaluate testosterone concentration by competitive sandwich ELISA assay with colorimetric method. The Lovetuner is calibrated to 528hz, a natural frequency that vibrates at the same frequency of our heart and our DNA. One of the chief effectors for relive of distress is production of testosterone in brain. The data were analyzed using one-way ANOVA. This suggestion may be counter-intuitive to what we do, creating music based on sound healing frequencies everyday. The POMS 2 consists of 35 items questions and a respondent answer each of question with the evaluation scale of POMS 2 (0 = not at all, 1 = slightly, 2 = modestly, 3 = considerably, 4 = very). In this study, we measured salivary biomarkers of stress (cortisol, chromogranin A, and oxytocin), which can be collected noninvasively, as indices of stress relief. The 9 Solfeggio frequencies are 174 Hz, 285 Hz, 396 Hz, 417 Hz, 528 Hz, 639 Hz, 741 Hz, 852 Hz and 963 Hz. The logic behind this is that ultimately only you can know how a certain frequency affects you. Furthermore, anger-hostility scores (indicating negative mood) slightly increased, and friendliness scores (indicating positive mood) decreased (Table 1). It is one of the most mysterious solfeggio frequencies which research has found to reduce stress and promote relaxation. We thank prof. B. Bolouri for technical assistance and data analysis. A p value of <0.05 was considered statistically significant. Furthermore, others report that listening to high-frequency music increases the activity of the parasympathetic nervous system and reduces stress, in comparison to listening to low-frequency music [3] . In other words, we could not confirm that a stress mitigation effect occurred in the 440 Hz music condition. The data were analyzed using one-way ANOVA. Will this be bad for e to listen to from my iPhone 8? The highest frequencies are the first to go as we become older. You might be wondering why it is that Solfeggio frequencies produce more positive effects on the body than any other sounds or tones. However, no significant change was observed in any salivary biomarkers in the 440 Hz condition. If you are around a person who is having a seizure or experiencing an epileptic fit, they would likely cause significant harm to your ears. The 528 Hz frequency is known for its link to the Solar Plexus Chakra. 10, 11). Mol Endocrinol 8:654662, Ingraham HA, Lala DS, Ikeda Y, Luo X, Shen W-H, Nachtigal MW, Abbud R, Nilson JH, Parker KL (1994) The nuclear receptor steroidogenic factor 1 acts at multiple levels of the reproductive axis. Interestingly, after listening to 440 Hz music, mean levels of chromogranin A showed a tendency to increase, while LF/HF significantly decreased in both conditions. The total testosterone was evaluated with ELISA Kit (Abcam, ab108666). United States patent US 9,107,389. These Solfeggio frequencies were believed to profoundly affect the conscious and subconscious mind in order to stimulate healing and promote vitality. Add your e-mail address to receive free newsletters from SCIRP. Colloids Surf B 27:287293, Luo X, Ikeda Y, Parker KL (1994) A cell-specific nuclear receptor is essential for adrenal and gonadal development and sexual differentiation. 2007). al. Ayahuasca Legal Status: Community Medicine or Deep State New Religion? No, it seems that the 528hz claim is either completely made up, or at best based off of recordings artificially adjusted to be heard by humans. This assay is conducted by the measurement of cytoplasmic lactate dehydrogenase enzyme (LDH), which is present in cell culture and upon damage to plasma membranes are by apoptosis or other factors, this enzyme is released to the medium. Combine the different features and mix them together to create your own perfect sleep sanctuary! An electrocardiogram could not be recorded correctly for one of the participants, so we analyzed the data from eight participants for this measure (Figure 5). People who are exposed to these sources may experience dizziness or nausea if they are sensitive to the noise."}}]}. Is this not the case with your Solfeggio frequency? All of the frequencies mentioned can be found in the BetterSleep app to mix with other sounds or content. Whereas the control group was treated in the same manner, without any exposure to sound waves. By continuing to browse this site, you agree to this use. Our mental state is determined by the prevailing frequency, and entrainment momentarily modifies our mental state by making one frequency louder than the others. Dont let frequencies decide what feels good and uplifting for you. knitting for charity glasgow. So if you tune your violin and for example to 440 Hz, then you are tuning the A string so that when played, IT vibrates at a frequency of 440 oscillations per second. Tinnitus is a high-pitched sound produced by hearing loss in the high-frequency range, and a low-pitched sound produced by hearing loss in the low-frequency range. It also aids in awakening intuition and inner strength. Thats the sound of a phone ringing in your ears all the time. I looked it up on handy google and this was correct. Throughout the years, weve seen more and more music healers choosing to tune in 528 Hz for awakening spirituality. Figure 1. Thank you. So the sound wave could not alter the LDH activity. Since the early 1990s, a curious thing has been happening in the world of alternative health and healing. To do (c), tell someone that you have transposed it to 528 Hz. wellness. Salivary samples were collected at five time points: immediately before music exposure, immediately after music exposure, 15 minutes later, 30 minutes later, and 45 minutes later. So I messaged the developer and later found out that the hz converter is always to the standard A. The threshold for death is commonly set at roughly 185-200 decibels, while 150 decibels is usually regarded enough to shatter your eardrums. Here is 528Hz Sleep Music track from Meditative Minds YT Channel, we hope this will help you. 1963). GAPDH primer pair was used as internal control. The data were analyzed using one-way ANOVA. Music of the aforementioned frequency (528 Hz) has lately attracted attention as healing music. 528 Hz - love and DNA repair, believed to bring about miracles. This HZ is so dangerous that the Rockefeller foundation actually did their best to BAN it from music as a whole to shut down the right brain of the population, because when 528 HZ is enacted the right brain opens and the heart-mind connection of the body strengthens drastically. And in a study published in the Journal of Addiction Research & Therapy, the frequency of 528 Hz reduced the toxic effects of ethanol, the principle ingredient found in alcoholic drinks, on cells. For an example of 528 hertz sound therapy, see the video here . Cells were washed twice with PBS-EDTA (10mM in PBS) (pH 7.4) and re-suspended in 0.5% trypan blue (1mL), and the number of viable cells was counted by hemacytometer. High frequency noises include loud whistles, squeaks, and squeals, as well as a childs voice. Fluorescence spectroscopy was monitored by microplate at excitation of 485nm and emission of 538nm. Hi meditative mind, In his research, he used mathematical numeral reduction to identify six measurable tones that bring the body back into balance and aid in healing. Many Regards, Dilpreet@MeditativeMind. Then cells were washed and incubated in medium. J Radiat Res 45:479489, Fukui H (2001) Music and testosterone. In contrast, after exposure to 440 Hz music, almost all scores showed a similar tendency to change in the same direction as observed in the 528 Hz music condition, but there was no significant difference for any score. LinkedIn Oct 25, 2017. He studied the connection between the Schumann resonances and found them to match various levels of human brain activity by comparing EEG recordings with the Earths electromagnetic fields. When an individual hears music, responses to these stimuli are eventually transferred to the amygdaloid body via the medial geniculate body of the thalamus. The energizing and healing effects of music set to 528 Hz make it ideal background music any time you feel under the weather or simply need to unwind. p values<0.05 were considered statistically significant. How to Remove Low-Frequency Noise from a Room. You can even play the frequencies in the background during your normal daily activities (except potential dangerous activities like driving etc.). The results were defined and expressed as a percentage of viability of control group. This measure represents the coefficient of variance of the R-R interval (RRI) and is calculated by dividing the standard deviation of the RRI by the mean of RRI. 59 7 Bob Singer Works at NASA Author has 6.2K answers and 9.8M answer views 5 y Cells cultures were loaded with 10mM DCDHF in serum free medium (DEMEM) for 30min at 37C. 100dB intensity reduced ROS production in 520 and 530Hz frequency rather than other frequency. It is one of the most mysterious solfeggio frequencies which research has found to reduce stress and promote relaxation. 2002). Therefore we used LDH assay for further analysis of cell viability. Points of coupling of four different tetrazolium salts. The viability of cells could be inversely affected by overproduction of ROS. And this, in turn, tunes the other strings so that they are in equal tempered tuning with the A string. Low frequency noises include a bass drum, a rumble, thunder, and a mans deep voice. obsolete thinking by 528 Hz published on 2021-11-21T05:17:14Z. J Clin Endocrinol Metab 90:893903, Heinlein CA, Chang C (2002) The roles of androgen receptors and androgen-binding proteins in nongenomic androgen actions. Tambin trae alegra, energa vital, claridad a la mente, autoconciencia, creatividad y gua divina relacionada con el Chakra del Plexo Solar. Musically speaking, the frequencies are derived by beginning at 8Hz and working up the musical scale octave by octave until the C note is vibrating at the 256 Hz frequency and the A note is vibrating at 432 Hz. Time points are as indicated in Figure 1 (Mean SE) *p < 0.05. hypothalamic-pituitary-adrenal (HPA) axis in response to stress. Use the Lovetuner and Connect to the 528hz Frequency, the so-called Love Frequency, Lovetuner We use cookies for analytics, personalized content and ads. However, you can still decide which notes you want to tune to 528 Hz. The Ca2+ concentration in cell culture after 1h treated with different intensities of 528Hz sound wave: Data are shown as mean SEM of three separate experiments. Accordingly, we examined the effect of such music compared to 440 Hz music. Oxford University Press, Oxford, Kamogashira T, Fujimoto C, Yamasoba T(2015) Reactive oxygen species, apoptosis, and mitochondrial dysfunction in hearing loss. You should always take extra care when moving into these situations! Therefore it appears that exposure to 528Hz frequency with 100dB intensity increases testosterone biosynthesis in brain by increasing of SF-1 level, improves its transportation of cholesterol through mitochondrial membrane and also retards its degradation by reducing level of P450 aromatase gene. When music is tuned to harmonize with this frequency it is known as scientific tuning. The 432 Hz frequency resonates with the Schumann Resonance of 8 Hz and is known for its deeply calming and soothing effects. Therefore, at distance of 20cm, frequency of 528Hz did not induce sufficient energy to disrupt cell membrane integrity (Fig. Although the sample size of this study was limited, these are interesting data and could provide support for a new approach in music therapy. All authors reviewed the manuscript. Effects of music on salivary oxytocin. It can cause sensations of sorrow, coldness, anxiety and even shivers down the spine. This Ca2+ influx through voltage-gated channels changes the action potential of astrocyte cell and it serves as the second messenger for initiation of many different cellular events. Music, Stress, Salivary Assay, Autonomic Nervous System. It cant be seen or heard; it may happen inside or outdoors; its not biological, environmental, or radioactive, yet it can make you sick or kill you. Stop listening to distressing music in whatever genre. Therefore this frequency was chosen for further analysis. Humans can detect noises at frequencies ranging from 20 to 20,000 cycles per second, or hertz (Hz), however this range narrows with age. The Open Arm time (OAT) and Open Arm Entrance (OAE) variation calculated by following formula (Eqs. Listening to music tuned to this frequency is most helpful when you want to feel more uplifted, secure, and give power to your goals and dreams. Various effects have been ascribed to the solfeggio frequency, but none of these have any scientific basis. J Mol Endocrinol 29:2339, dos Reis Lestard N, Valente RC, Lopes AG, Capella MA (2013) Direct effects of music in non-auditory cells in culture. We have seen that at lower numbers, youtube converts stereo audio signal to mono. Its found in nature and oxygen, rainbows, grass and chlorophyll, sun rays as well as in the buzzing of bees. The different intensity of 528Hz sound wave significantly reduced ROS production in brain. Time points are as indicated in Figure 1 (Mean SE) *p < 0.05. The effect of this frequency was remarkable and the production of ROS was significantly reduced at 100dB intensity. J Addict Res Ther 8:2, Biggio G, Purdy RH(2001) Neurosteroids and brain function. Create a SoundCloud account An hour long, pure tone meditation soundtrack at the healing frequency of 528hz. What sound frequency is detrimental to people in this case? For example, it has been applied to the course of treatment of mental disorders, mental changes of people with physical disorders, screening in the workplace, and exercise or relaxation effect. Find a quiet and comfortable place where you can sit or lie down. The concentrations of enzyme in the media were determined by direct calculation based upon the decreases in absorbance. According to Dr. Horowitz, this is why grass is green. Box 13145-1384, Tehran, Iran, T. Babayi Daylari,G. H. Riazi,Sh. According to scientific research, cancer is not only caused by mobile phone radiation, and that other variables may also play a role in its development. Some researchers report that, depending on the mental state of the listener, music preferences vary [13] . There's a harvard study says grey matter growth after 8 weeks of meditation, also some studies on 528 Hz reducing stress, cortisol. 3). The cell morphology changed to confocal after 11days. 417 Hz - helps to deal with change and removes old patterns and habits. How to Cope with Holiday Depression and Stress, mental health If you are asking if its harmful to any other living organism (that I know of) the answer is still no, but don't quote me. the incredible similarities between the Schumann resonance and human brain activity. All Rights Reserved. Mol Endocrinol 16:21812187, Horowitz LG (2000) Healing codes for the biological apocalypse. The primers were produced locally from Pishgam Tehran (Iran). Aside from the aforementioned, can high-frequency noises harm you? THE FREQUENCIES FROM 396HZ TO 852HZ ARE A DANGEROUS HOAX - DANGEROUS TO BOTH YOUR BODY AND BRAIN. In this musical scale, there is no 528 Hz note. 852 Hz - awakens, allows you to see the truth, develops intuition. Despite its growing popularity, there is still a lot we dont know about the 528Hz frequency. Time points are as indicated in Figure 1 (Mean SE). It is possible to quickly evaluate not only the state of emotion that lasts for a relatively long time but also the state of temporary feelings and emotions that change depending on the situation that subject is in. He has an interesting take on the power of 528 Hz and how it affects every living thing in the universe. It has been demonstrated that sound waves could have either a constructive or distractive effect on cellular viability. Even though the science on the reparative effects of the 528 Hz is still in its infancy, early research indicates that it has the ability to heal and repair the body. The different Solfeggio Frequencies are: 174 Hz : For the relief of Pain & Stress. Significant difference between treatment and control group at p0.05 are presented as *. In 1988, biochemist Dr. Glen Rein made a discovery that confirmed what the ancient spiritual traditions understood when he tested the impact of different music on human DNA. Newsmatics Schumanns successor, doctoral candidate Herbert Konig, took this research a step further. More than any sound previously discovered, the "LOVE frequency" resonates at the heart of everything. As mentioned earlier, the 528 Hz frequency has not been known to be harmful to people at any point in time. A bass boost that is too strong might make the music seem boomy. Mean levels of chromogranin A showed a tendency to decrease after listening to 528 Hz music. Your help is welcome. COVID Nuremberg Trial Advancing Against W.H.O. The low frequency sound effects on humans is a question that has been asked for a long time. In this article, well take a look at what we know (and dont know) about this curious phenomenon and this mysterious frequency. The Solfeggio frequencies include, but are not limited to, six different tones. music tuned to 432 Hz slows down the heart rate, music tuned to the frequency of 528 Hz significantly reduced stress in the endocrine systems and autonomic nervous systems, 528 Hz reduced the toxic effects of ethanol. These frequencies are reputed to date back to ancient history and said to be the fundamental sounds used in both Western Christianity and Eastern Indian religions, chanted by the Gregorian Monks and in ancient Indian Sanskrit chants. 1st time in months by 528 Hz published on 2021-03-08T05:46:06Z. The significance of sound waves on key enzymes that regulate levels of testosterone in brain has not been investigated. / Copyright 2023 by authors and Scientific Research Publishing Inc. 528Hz frequency is believed to have profound effects on the body and mind. / Gen Comp Endocrinol 139:7284, Guo W, Bachman E, Li M, Roy CN, Blusztajn J, Wong S, Chan SY, Serra C, Jasuja R, Travison TG (2013) Testosterone administration inhibits hepcidin transcription and is associated with increased iron incorporation into red blood cells. 4. Prolonged exposure to this sound wave showed reduction of anxiety related behaviors in rats. We do not allow opaque clients, and our editors try to be careful about weeding out false and misleading content. Chromogranin A is found in the duct of the submandibular gland. the Schumann resonance has been found to provide synchronization for higher brain function. After conjugation of cytoplasmic Ca2+ to mitochondrial membrane steroidogenic acute regulatory protein (StAR), uptake of cholesterol into preplasmic space will be increased. We measured cortisol, chromogranin A, and oxytocin as salivary biomarkers of stress. Really dont mind paying a price, just that there are times when Im not online etc.. How can one determine if one is receiving youtube in HD? Everyone participated with full understanding of the study and gave their informed consent. The astrocyte cells were exposed to 1h to sound waves with a frequency of 528Hz and different intensity (80, 100 and 120dB) in a distance from the ground to avoid possible vibration. Brain tissue were obtained from sacrificed rats at each time points, immediately immersed in liquid nitrogen and stored at 80 C until analyzed. At this time, medium was removed, cells were washed with DMEM and 1ml of trypsinEDTA (Gibco) (0.25%) solution was added and incubated for 5min. The animals were randomly divided into four groups (three groups for 80, 100 or 120dB, respectively) and a control group. Mean levels of oxytocin also increased after listening to 440 Hz music, but no significant difference was identified. Exp Neurol 181:301312, Bustin S (2002) Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. Noise Health 15:307, Drge W (2002) Free radicals in the physiological control of cell function. Data are shown as mean SEM of three separate experiments. Further research supports Konigs findings that confirm the incredible similarities between the Schumann resonance and human brain activity. You will find the effects associated with each slider in the preset list. 528 Hz - Transformation and Miracles (DNA Repair) 639 Hz - Connecting/Relationships 741 Hz - Expression/Solutions 852 Hz - Returning to Spiritual Order In fact, the series is not based on any actual musical, aesthetic or acoustic considerations, only numerological ones. 2 g of total RNA was reverse-transcribed at 50 C for 30 min in a reaction mixture which contains 0.5 mM dNTP, 400U of MMLV Reverse Transcriptase, 1RT buffer, 0.5 mM dithiotreitol and 0.5 g of oligo dT. It can cause sensations of sorrow, coldness, anxiety and even shivers down the spine. Is 528 Hz a safe frequency? Additionally, all positive mood scores tended to increase. 528 Hz is essential to the geometry of circles and spirals consistent with DNA structuring and hydrosonic restructuring. The mRNA level for P450 aromatase gene, which is involved with destruction of testosterone, remained steady at 80 and 120dB intensities however it was decreased significantly at 100dB (Fig. 528 Hz frequency is believed to be so powerful that it can help repair DNA damage, bring peace and harmony, and restore equilibrium to everything around it. Humans can generally perceive noises in the 20-20,000 Hz range, and it is widely recognized that frequencies in this range may cause hearing impairment. Incredibly, that precise frequency of 528Hz is encrypted in the Bible's Book of Numbers as the most valuable 'gold offering' to God used to sanctify the templein this case the 'body temple.' 528Hz. Concentration of testosterone in brain tissue: testosterone concentration in exposed brain tissue to sound wave had significantly different with control groups after 21days. This suggests that participants felt comfortable listening to 528 Hz music and this stimulus reduced their stress level. After 21day of treatment with 528Hz frequency at various intensities, Rats are tested in EPM and data related to time and number of entrance to each arm in 5min was enumerated. All experiments were conducted along with the institutional ethical guidelines. Specifically, they report that salivary cortisol and chromogranin A decrease significantly before and after listening to music [8] . Astrocytes have many channels and receptors that are responsible for increasing the intracellular calcium concentration (Pasti et al. Examples: The frequency of 528 Hz supposedly has healing effects on DNA. And rock music decreased UV light absorption, harming the DNA. Then measurement was performed at 340nm. Were all different. The results shown that upon exposure to sound frequencies of 500 and 600Hz, the ROS production was noticeably reduces (Fig. Youll enjoy listening to music set to 852 Hz if youre seeking to communicate with and live in harmony with your highest self. Time points are as indicated in Figure 1 (Mean SE) *p < 0.05. Dissociation curve analysis was performed after each assay to determine target specificity. With over 10 years of experience teaching others about inner peace and how to achieve it with ease, we hope to share that knowledge on Nature Sound Therapy for you to enjoy for yourself. Chris Levine, 528 Hz Love Frequency at Houghton Hall Thursday, Friday, Saturday evenings 4:30- 9pm until 23 December 2021 Tickets: Adults 18 Under 18s free and concessions for students. Research has investigated many aspects of this phenomenon, and it is recognized that several constituent elements of music are involved in this stress reduction effect. It is well known that listening to music reduces stress. A: Yes, low-frequency sound can be made by machinery in industrial and construction settings. 1 and 2): Astrocyte primary cell cultures were placed at distance of 20cm under the sound waves with frequencies between 100 and 800Hz and intensity ofdB and then production of ROS was determined. Other frequency the geometry of circles and spirals consistent with DNA structuring and hydrosonic restructuring G... A tendency to decrease after listening to music set to 852 Hz if youre to. And testosterone your e-mail address to receive free newsletters from SCIRP you want to tune 528. High-Frequency noises harm you he has an interesting take on the body and brain function after assay. Health 15:307, Drge W ( 2002 ) Quantification of mRNA using real-time reverse transcription PCR ( RT-PCR ) trends! A certain frequency affects you than other frequency of the frequencies in the 440 Hz.! Its found in the world of alternative health and healing Minds YT Channel, we hope will. 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